Skeletal Biology and Disease Modeling in Zebrafish.

Zebrafish are teleosts (bony fish) that share with mammals a common ancestor belonging to the phylum Osteichthyes, from which their endoskeletal systems have been inherited. Indeed, teleosts and mammals have numerous genetically conserved features in terms of skeletal elements, ossification mechanisms, and bone matrix components in common. Yet differences related to bone morphology and function need to be considered when investigating zebrafish in skeletal research. In this review, we focus on zebrafish skeletal architecture with emphasis on the morphology of the vertebral column and associated anatomical structures. We provide an overview of the different ossification types and osseous cells in zebrafish and describe bone matrix composition at the microscopic tissue level with a focus on assessing mineralization. Processes of bone formation also strongly depend on loading in zebrafish, as we elaborate here. Furthermore, we illustrate the high regenerative capacity of zebrafish bones and present some of the technological advantages of using zebrafish as a model. We highlight zebrafish axial and fin skeleton patterning mechanisms, metabolic bone disease such as after immunosuppressive glucocorticoid treatment, as well as osteogenesis imperfecta (OI) and osteopetrosis research in zebrafish. We conclude with a view of why larval zebrafish xenografts are a powerful tool to study bone metastasis. © 2021 American Society for Bone and Mineral Research (ASBMR).

Two millennia of North Atlantic seasonality and implications for Norse colonies.

delta(18)O values of mollusks recovered from near-shore marine cores in northwest Iceland quantify significant variation in seasonal temperature over the period from approximately 360 B.C. to approximately A.D. 1660. Twenty-six aragonitic bivalve specimens were selected to represent intervals of climatic interest by using core sedimentological characteristics. Carbonate powder was sequentially micromilled from shell surfaces concordant with growth banding and analyzed for stable oxygen (delta(18)O) and carbon (delta(13)C) isotope values. Because delta(18)O values record subseasonal temperature variation over the lifetime of the bivalves, these data provide the first 2,000-year secular record of North Atlantic seasonality from ca. 360 cal yr B.C. to cal yr A.D. 1660. Notable cold periods (360 B.C. to 240 B.C.; A.D. 410; and A.D. 1380 to 1420) and warm periods (230 B.C. to A.D. 140 and A.D. 640 to 760) are resolved in terms of contrast between summer and winter temperatures and seasonal temperature variability. Literature from the Viking Age (ca. 790 to 1070) during the establishment of Norse colonies (and later) in Iceland and Greenland permits comparisons between the delta(18)O temperature record and historical records, thereby demonstrating the impact of seasonal climatic extremes on the establishment, development, and, in some cases, collapse of societies in the North Atlantic.